Antagonists of Wnt/-catenin signalling in the periodontitis associated with type 2 diabetes and smoking

作者：Miranda Tamires S; Napimoga Marcelo H; Feres Magda; Marins Leticia M; da Cruz Daniele F; da Silva Helio Doyle P; Duarte Poliana M^*

来源：Journal of Clinical Periodontology, 2018, 45(3): 293-302.

DOI：10.1111/jcpe.12854

• chronic periodontitis
• diabetes mellitus
• DKK1
• sclerostin
• smoking

AimThis study evaluated the levels of sclerostin (SOST) and Dickkopf (DKK)-1 in the chronic periodontitis (CP) associated with type 2 diabetes (DM) and/or smoking. Relationships between SOST, DDK1, RANKL, OPG, IL-1 ss, IL-6 and TNF-, and pathogens were assessed.
Material and MethodsThe study population included non-diabetic non-smokers (control), non-smokers with DM (DM group), non-diabetic smokers (S group) and smokers with DM (SDM group), all with CP. Serum and gingival levels of SOST, DKK1, RANKL, OPG, IL-1 ss, IL-6 and TNF- were evaluated by multiplex immunoassay. Gene expressions of these biomarkers and subgingival levels of pathogens were assessed by qPCR.
ResultsGingival protein and/or mRNA levels of DKK1 and SOST were higher in subjects with DM and/or smoking than in controls (p<.05). Serum levels of SOST were higher in the DM group than in controls (p<.05). DKK1 positively correlated with SOST in the DM, SDM and control groups (p<.05) at mRNA levels. DKK-1 and SOST correlated with pathogens, especially in both groups with DM.
ConclusionsSOST and DKK1 were upregulated in patients with CP presenting DM and/or smoking. DM, alone or with smoking, particularly influenced the correlations of SOST and DKK1 with each other and with the other biomarkers mostly at mRNA levels, as well as with periodontal pathogens.

• 出版日期2018-3