LIMP-2 Links Late Phagosomal Trafficking with the Onset of the Innate Immune Response to Listeria monocytogenes A ROLE IN MACROPHAGE ACTIVATION

作者：Carrasco Marin Eugenio^*; Fernandez Prieto Lorena; Rodriguez Del Rio Estela; Madrazo Toca Fidel; Reinheckel Thomas; Saftig Paul; Alvarez Dominguez Carmen

来源：Journal of Biological Chemistry, 2011, 286(5): 3332-3341.

DOI：10.1074/jbc.M110.146761

The innate immune response to Listeria monocytogenes depends on phagosomal bacterial degradation by macrophages. Here, we describe the role of LIMP-2, a lysosomal type III transmembrane glycoprotein and scavenger-like protein, in Listeria phagocytosis. LIMP-2-deficient mice display a macrophage-related defect in Listeria innate immunity. They produce less acute phase pro-inflammatory cytokines/chemokines, MCP-1, TNF-alpha, and IL-6 but normal levels of IL-12, IL-10, and IFN-gamma and a 25-fold increase in susceptibility to Listeria infection. This macrophage defect results in a low listericidal potential, poor response to TNF-alpha activation signals, impaired phago-lysosome transformation into antigen-processing compartments, and uncontrolled LM cytosolic growth that fails to induce normal levels of acute phase pro-inflammatory cytokines. LIMP-2 transfection of CHO cells confirmed that LIMP-2 participates in the degradation of Listeria within phagosomes, controls the late endosomal/lysosomal fusion machinery, and is linked to the activation of Rab5a. Therefore, the role of LIMP-2 appears to be connected to the TNF-alpha-dependent and early activation of Listeria macrophages through internal signals linking the regulation of late trafficking events with the onset of the innate Listeria immune response.

• 出版日期2011-2-4