Application of high-throughput, high-resolution and cost-effective next generation sequencing-based large-scale HLA typing in donor registry

作者：Zhou, M.; Gao, D.; Chai, X.; Liu, J.; Lan, Z.; Liu, Q.; Yang, F.; Guo, Y.; Fang, J.; Yang, L.; Du, D.; Chen, L.; Yang, X.; Zhang, M.; Zeng, H.; Lu, J.; Chen, H.; Zhang, X.; Wu, S.; Han, Y.; Tan, J.; Cheng, Z.; Huang, C.; Wang, W.^*

来源：Tissue Antigens, 2015, 85(1): 20-28.

DOI：10.1111/tan.12477

• allelic level
• cost effective
• genotyping
• high throughput
• human leukocyte antigen
• next generation sequencing

Next generation sequencing (NGS)-based human leukocyte antigen (HLA) typing was used for ultra large-scale genotyping of registry donors for the China Marrow Donor Program (CMDP). More than 79,000 samples were subjected to HLA genotyping at 4-digit allelic level without ambiguities for HLA-A, -B, -C, DRB1 and DQB1 loci, with throughput up to 2068 samples per lane in a HiSeq flow cell (eight lanes per run), and cost reduced by 95% compared with that of Sanger-based typing. Two percent of randomly selected samples were quality control (QC) tested at 4-digit allelic level by the CMDP QC laboratory, yielded a concordance of 99.72%. These results demonstrate that NGS is a cost effective and valuable tool for HLA typing of registry donors.

• 出版日期2015-1