Arterial Baroreflex Dysfunction Impairs Ischemia-Induced Angiogenesis

作者：Hao, Changning; Huang, Zhen-Hao; Song, Shu-Wei; Shi, Yi-Qin; Cheng, Xian Wu; Murohara, Toyoaki; Lu, Wei^*; Su, Ding-Feng; Duan, Jun-Li

来源：Journal of the American Heart Association, 2014, 3(3): e000804.

DOI：10.1161/JAHA.114.000804

• acetylcholinesterase inhibitor
• angiogenesis
• arterial baroreflex
• non-neural cholinergic system
• peripheral vascular disease

Background-Endothelium-derived acetylcholine (eACh) plays an important role in the regulation of vascular actions in response to hypoxia, whereas arterial baroreflex (ABR) dysfunction impairs the eACh system. We investigated the effects of ABR dysfunction on ischemia-induced angiogenesis in animal models of hindlimb ischemia with a special focus on eACh/nicotinic ACh receptor (nAChR) signaling activation.
Methods and Results-Male Sprague-Dawley rats were randomly assigned to 1 of 3 groups that received (1) sham operation (control group), (2) sinoaortic denervation (SAD)-induced ABR dysSAD group), or (3) SAD rats on diet with an acetylcholinesterase inhibitor pyridostigmine (30 mg/kg per day, SAD+Pyr group). After 4 weeks of the SAD intervention, unilateral limb ischemia was surgically induced in all animals. At postoperative day 14, SAD rats exhibited impaired angiogenic action (skin temperature and capillary density) and decreased angiogenic factor expressions (vascular endothelial growth factor [VEGF] and hypoxic inducible factor [HIF]-1 alpha) in ischemic muscles. These changes were restored by acetylcholinesterase inhibition. Rats with ABR dysfunction had lower eACh levels than did control rats, and this effect was recovered in SAD+Pyr rats. In alpha 7-nAChR knockout mice, pyridostigmine improved ischemia-induced angiogenic responses and increased the levels of VEGF and HIF-1 alpha. Moreover, nicotinic receptor blocker inhibited VEGF expression and VEGF receptor 2 phosphorylation (p-VEGFR2) induced by ACh analog.
Conclusions-Thus, ABR dysfunction appears to impair ischemia-induced angiogenesis through the reduction of eACh/alpha 7-nAChR-dependent and -independent HIF-1 alpha/VEGF-VEGFR2 signaling activation.

• 出版日期2014-6
• 单位中国人民解放军第二军医大学; 上海交通大学; 中国科学院; 中国科学院上海技术物理研究所