Acute toxicity test using cyanide on Daphnia magna by flow-through system

作者：Jaafarzadeh N^*; Hashempour Y; Angali K Ahmadi

来源：Journal of Water Chemistry and Technology, 2013, 35(6): 281-286.

DOI：10.3103/S1063455X13060076

• acute toxicity test
• bioassay
• Daphnia magna
• sodium cyanide

This study describes the acute toxicity of sodium cyanide on the crustacean Daphnia magna (Cladocera). A new flow-through system was innovated in which the microorganism continuously exposure to the toxicity of sodium cyanide during the test. We used twelve different concentration of sodium cyanide from low values (0 mg CN/L) to high values (1 mg CN/L) on bioassay test. D. magna were exposed to the concentrations for 24, 48, 72 and 96 h at 20-25A degrees C. We controlled the three important parameters such as temperature, pH and DO to meet the standard requirements. The LC50 values for 24, 48, 72 and 96 h (95% confidence limits in parentheses) were estimated statistically by the probit methods and were 0.171 (0.163-0.179), 0.12 (0.112-0.128), 0.07 (0.062-0.078) and 0.019 (0.011-0.027) mg/L respectively. Finally, we proposed two new values for SAR (safe application rate) and SAFE Coefficients.

• 出版日期2013-11