Wild blueberry consumption attenuates local inflammation in the perivascular adipose tissue of obese Zucker rats

作者：Vendrame Stefano; Tsakiroglou Panagiotis; Kristo Aleksandra S; Schuschke Dale A; Klimis Zacas Dorothy^*

来源：Applied Physiology Nutrition and Metabolism, 2016, 41(10): 1045-1051.

DOI：10.1139/apnm-2016-0160

• PVAT
• blueberries
• anthocyanins
• metabolic syndrome
• inflammation
• Zucker rat
• endothelial function
• nitric oxide synthase
• cyclooxygenase
• MCP-1
• TNF-alpha

Perivascular adipose tissue (PVAT) has been shown to play important roles in regulating vascular tone and linking local and systemic vascular inflammation. We examined the impact of PVAT on phenylephrine-mediated vasoconstriction in the aorta of obese Zucker rats (OZR) and their lean littermates (LZR) by comparing aortic rings with or without PVAT. Subsequently we placed OZR and LZR on a control (C) or an 8% wild blueberry (WB) diet and evaluated the effect of WB consumption on such response. PVAT-released adipokine concentrations were also measured as a function of WB diet. Maximal constrictor force (Fmax) in aortic rings without PVAT was significantly lower in OZR-C compared with LZR-C (0.41 +/- 0.05 and 0.71 +/- 0.06 g, respectively). Following WB diet, Fmax significantly increased in OZR (0.54 +/- 0.06 g). In aortas with intact PVAT, Fmax was significantly lower in all groups (0.31 +/- 0.06 OZR-C, 0.30 +/- 0.05 OZR-WB, 0.29 +/- 0.03 LZR-C, and 0.30 +/- 0.04 g LZR-WB), but no difference was observed between treatments. PVAT concentrations of monocyte chemoactractant protein 1 (MCP-1), tumor necrosis factor alpha, and adiponectin were significantly higher in OZR compared with LZR (+102%, +108%, and +45%, respectively). Following WB diet, PVAT concentrations of interleukin-8 were significantly lower in both OZR (-37%) and LZR (-30%), while adiponectin concentrations significantly increased in both OZR (+11%) and LZR (+16%). MCP-1 concentrations significantly decreased (-31%) in the PVAT of OZR with the WB diet. WB consumption appears to attenuate local inflammation in PVAT, which may impact systemic vascular inflammation and endothelial function.

• 出版日期2016-10