A Peptide Nucleic Acid (PNA) Heteroduplex Probe Containing an Inosine-Cytosine Base Pair Discriminates a Single-Nucleotide Difference in RNA

作者：Matsumoto Katsuhiko; Nakata Eiji; Tamura Tomoki; Saito Isao; Aizawa Yasunori; Morii Takashi^*

来源：Chemistry - A European Journal, 2013, 19(16): 5034-5040.

DOI：10.1002/chem.201204183

• DNA
• fluorescent probes
• peptide nucleic acids
• RNA
• sequence determination

Selective discrimination of a single-nucleotide difference in single-stranded DNA or RNA remains a challenge with conventional DNA or RNA probes. A peptide nucleic acid (PNA)-derived probe, in which PNA forms a pseudocomplementary heteroduplex with inosine-containing DNA or RNA, effectively discriminates a single-nucleotide difference in a closely related group of sequences of single-stranded DNA and/or RNA. The pseudocomplementary PNA heteroduplex is easily converted to a fluorescent probe that distinctively detects a member of highly homologous let-7 microRNAs.

• 出版日期2013-4