Analysis of the Biological Function of ELDF15 Using an Antisense Recombinant Expression Vector

作者：Liu Yan; Wang Long; Wang Zi Jun^*

来源：Asian Pacific Journal of Cancer Prevention, 2014, 15(21): 9131-9136.

DOI：10.7314/APJCP.2014.15.21.9131

• ELDF15
• gene
• cell proliferation
• differentiation
• antisense technology
• carcinogenesis

ELDF15, homologous with AT2 receptor-interaction protein 1 (ATIP1), may play an important role in cell differentiation, proliferation, and carcinogenesis. We aimed to understand the biological function of ELDF15 via construction and transfection of a recombinant expression vector containing antisense ELDF15. Recombinant expression vectors were successfully constructed and transfected into K562 cells. A stable transfectant, known as pXJ41-asELDF15, stably produced antisense ELDF15. Compared with K562 and K562-zeo cells, K562-pXJ41-asELDF15 cells showed inhibition of cell proliferation. RT-PCR analysis showed that the expression and protein level of ELDF15 decreased significantly in K562 cells transfected with pXJ41-asELDF15. Expression of hemoglobin increased in K562 cells transfected with pXJ41-asELDF15 by benzidine staining. increases NBT reduction activity in K562 cells transfected with pXJ41-asELDF15. Colony forming efficiency in two-layer soft agar was clearly inhibited as assessed by electron microscopy. These results suggest that ELDF15 plays a potential role in cell differentiation, proliferation and carcinogenesis.

• 出版日期2014
• 单位中国疾病预防控制中心