Antioxidant activity of polysaccharide purified from Acanthopanax koreanum Nakai stems in vitro and in vivo zebrafish model

作者：Kang Min Cheol; Kim Seo Young; Kim Eun A; Lee Ji Hyeok; Kim Young Sun; Yu Seok Kyu; Chae Jun Byoung; Choe In Hu; Cho Jin Hun; Jeon You Jin^*

来源：Carbohydrate Polymers, 2015, 127: 38-46.

DOI：10.1016/j.carbpol.2015.02.043

• Acanthopanax koreanum Nakai
• Polysaccharide
• Enzyme-assistant extract
• Antioxidant activity
• Zebrafish
• Oxidative stress

In this study, the antioxidative effects of a purified polysaccharide isolated from the stems of Acanthopanax koreanum Nakai (ASP) on hydrogen peroxide-induced oxidative stress was investigated both in vitro and in vivo using a zebrafish model. A. koreanum Nakai stem was hydrolyzed using five carbohydrases and five proteases for the enzyme-assistant extraction. Of the enzyme-assistant extracts, the Protamex extract exhibited the highest yield and a potent scavenging activity against free radicals, Ethanol-added separation and anion exchange chromatography were conducted to identify the active polysaccharide. The purified polysaccharide significantly scavenged hydrogen peroxide and reduced hydrogen peroxide-induced cell death in Vero cells and in zebrafish. The results reveal that ASP is a useful antioxidant polysaccharide and might be available for relevant industrial applications.

• 出版日期2015-8-20