摘要

An ultra performance liquid chromatography (UPLC) method was developed for the simultaneous determination of psoralen, xanthotoxin and bergapten in Enrichment angelica pills and Angelica sinensis. The samples were extracted with methanol. The target compounds were seperated on Waters ACQUITY BEH C-18 (100 mm x 2.10 mm 1.7 mu m), with a gradient acetonitrile-water solvent system at 30 degrees C, the flow rate was 0.45 mL/min, the detection wavelength was set at 245 nm and the injection volume was 3 mu L. Results showed the good linear ranges for psoralen, xanthotoxin and bergapten were within 2.34-67.4 mg/L, with correlation coefficients(r) of 0.9992-0.9996, the mean recoveries were in the range of 96.20%-102.42%, with the relative standard deviation of 1.5%-3.0%. The method is simple, rapid, accurate and reliable, and suitable for the determination of psoralen, xanthotoxin and bergapten.

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