Anthocyanins from the fruits of Rubus croceacanthus and Rubus sieboldii, wild berry plants from Okinawa, Japan

作者：Kubota Michiyo; Ishikawa Chie; Sugiyama Yasumasa; Fukumoto Syuichi; Miyagi Tsuyoshi; Kumazawa Shigenori^*

来源：Journal of Food Composition and Analysis, 2012, 28(2): 179-182.

DOI：10.1016/j.jfca.2012.09.002

• Rubus croceacanthus
• Rubus sieboldii
• Anthocyanin
• Antioxidant activity
• ORAC
• Berry
• Okinawa
• Pigments in food
• Biodiversity
• Wild foods
• Food analysis
• Food composition

Anthocyanins from the fruits of Rubus croceacanthus and Rubus sieboldii, wild berries from Okinawa, Japan, were extracted using 80% methanol containing 0.5% acetic acid, and the extracts were separated by a DIAION HP-20 column. The major anthocyanins were purified by preparative HPLC and their structures were determined by NMR and MS spectrometry. Cyanidin-3-O-glucoside and pelargonidin-3-O-glucoside were identified from R. croceacanthus, and their contents were 81.44 +/- 1.01 mg and 170.02 +/- 4.36 mg/100 g fresh weight, respectively. Pelargonidin-3-O-glucoside and pelargonidin-3-O-rutinoside were identified from R. sieboldii, and their contents were 10.04 +/- 0.34 mg and 15.08 +/- 0.48 mg/100 g fresh weight,. respectively. An ORAC assay indicated that the two Okinawan wild berries, especially R. croceacanthus, had potent antioxidant activity compared to raspberry (Rubus idaeus).

• 出版日期2012-12