Novel Multiplex Fluorescent PCR-Based Method for HLA Typing and Preimplantational Genetic Diagnosis of beta-Thalassemia

作者：Khosravi Sharifeh; Salehi Mansour; Ramezanzadeh Mahboobeh; Mirzaei Hamed; Salehi Rasoul^*

来源：Archives of Medical Research, 2016, 47(4): 293-298.

DOI：10.1016/j.arcmed.2016.07.006

• Preimplantation genetic diagnosis
• beta-thalassemia
• HLA typing
• Multiplex fluorescent PCR
• STR markers

Background and Aims. Thalassemia is curable by bone marrow transplantation; however, finding suitable donors with defined HLA combination remains a major challenge. Cord blood stem cells with preselected HLA system through preimplantation genetic diagnosis (PGD) proved very useful for resolving scarce HLA-matched bone marrow donors.
Methods. A thalassemia trait couple with an affected child was included in this study. We used informative STR markers at the HLA and beta globin loci to develop a single cell multiplex fluorescent PCR protocol. The protocol was extensively optimized on single lymphocytes isolated from the couple's peripheral blood. The optimized protocol was applied on single blastomeres biopsied from day 3 cleavage stage IVF embryos of the couple.
Results. Four IVF embryos biopsied on day 3 and a single blastomere of each were provided for genetic diagnosis of combined beta-thalassemia mutations and HLA typing. Of these, one embryo was diagnosed as homozygous normal for the thalassemia mutation and HLA matched with the existing affected sibling.
Conclusion. The optimized protocol worked well in PGD clinical cycle for selection of thalassemia-unaffected embryos with the desired HLA system.

• 出版日期2016-5