A simple stripping voltammetric method for the determination of a new anticancer prodrug in serum

作者：Stepniowska Anna^*; Sztanke Malgorzata; Tuzimski Tomasz; Korolczuk Mieczyslaw; Sztanke Krzysztof

来源：Biosensors and Bioelectronics, 2017, 94: 584-588.

DOI：10.1016/j.bios.2017.03.053

• Anticancer prodrug
• ETTA
• Bismuth film electrode
• Adsorptive stripping voltammetry

The determination of ethyl [4-oxo-8-(3-chloropheny1)-4,6,7,8-tetrahydroimidazo[2,1-c][1,2,4]triazin-3-yl]acetate (ETTA), a new anticancer prodrug, using adsorptive stripping voltammetry (AdSV) was described for the first time. This method is based on adsorptive/reductive behaviour of ETTA at an in situ plated bismuth film electrode (BiFE) as a sensor. A number of experimental variables (e.g., a composition and pH of the supporting electrolyte, the conditions of bismuth film deposition, an accumulation potential and time, the scan rate, etc.) were thoroughly studied in order to achieve a high sensitivity. Experimental results under optimized conditions revealed an excellent linear correlation between the monitored voltammetric peak current and the ETTA concentration in the range of 2-50 mu g L-1 following an accumulation time of 300 s. The limit of detection (LOD) for ETTA following 300 s of an accumulation time was 0.4 mu g L-1. The proposed facile, sensitive and inexpensive method was successfully applied to the determination of ETTA in serum. The investigated prodrug was extracted from serum using SPE method.

• 出版日期2017-8-15