Design and synthesis of affinity chromatography ligands for the purification of 5-hydroxyeicosanoid dehydrogenase

作者：Reddy Chintam Nagendra; Ye Qiuji; Patel Pranav; Sivendran Sashikala; Chourey Shishir; Wang Rui; Anumolu Jaganmohan R; Grant Gail E; Powell William S; Rokach Joshua^*

来源：Bioorganic & Medicinal Chemistry, 2017, 25(1): 116-125.

DOI：10.1016/j.bmc.2016.10.017

• 5-HEDH
• 5-HETE
• 5-Oxo-ETE
• Eosinophil
• Neutrophil
• Affinity chromatography ligand
• Asthma

Arachidonic acid (AA) is converted to biologically active metabolites by different pathways, one of the most important of which is initiated by 5-lipoxygenase (5-LO). 5-Hydroxyeicosatetraenoic acid (5-HETE), although possessing only weak biological activity itself, is oxidized to 5-oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE), a potent chemoattractant for eosinophils and neutrophils. Our main goal is to determine how the biosynthesis of 5-oxo-ETE is regulated and to determine its pathophysiological roles. To achieve this task, we designed and synthesized affinity chromatography ligands for the purification of 5-hydroxyeicosanoid dehydrogenase (5-HEDH), the enzyme responsible for the formation of 5-oxo-ETE.

• 出版日期2017-1-1
• 单位McGill