Association Study Between the Single Nucleotide Polymorphisms of MUSTN1 Gene and Carcass Traits in Chickens

作者：Li, Juan; Wang, Cen; Kong, Fan-Li; Wang, Yan; Zhao, Xiao-Ling; Liu, Yi-Ping; Zhu, Qing^*

来源：Asian Journal of Animal and Veterinary Advances, 2012, 7(11): 1157-1165.

DOI：10.3923/ajava.2012.1157.1165

• Carcass traits
• chicken
• MUSTN1
• PCR-SSCP
• single nucleotide polymorphisms

MUSTN1 (Mustang, Musculoskeletal Temporally Activated Novel-1) gene is a musculoskeletal temporally activated novel gene which plays a key role in regulating the muscle development. In this study, the Single Nucleotide Polymorphisms (SNPs) in MUSTN1 were scanned, the association of SNPs With carcass traits in chickens were analyzed by Polymerase Chain Reaction-single-strand Conformation Polymorphism (PCR-SSCP) and DNA sequencing methods. Two variations (2120T>C and g.2160A>G) were identified when screened its entire exons and partial introns in 638 chickens. The genotypes of g.2120T>C (Mutations in exon 2) were significantly associated with breast muscle weight, muscle fiber diameters (p<0.05). The genotypes of g.2160A>G (Mutations in exon 2) were significantly associated with breast muscle weight, breast muscle weight percentage (p<0.05) and great significantly associated with muscle fiber diameters (p<0.01). MUSTN1 may act as a candidate gene of quantitative trait loci in regulating muscle growth.

• 出版日期2012-11
• 单位四川农业大学