alpha 6-Containing GABA(A) Receptors Are the Principal Mediators of Inhibitory Synapse Strengthening by Insulin in Cerebellar Granule Cells

作者：Accardi Michael V; Brown Patricia M G E; Miraucourt Lois S; Orser Beverley A; Bowie Derek^*

来源：Journal of Neuroscience, 2015, 35(26): 9676-9688.

DOI：10.1523/JNEUROSCI.0513-15.2015

• inhibition
• insulin
• metabolism
• mitochondria
• plasticity mechanism
• reactive oxygen species

Activity-dependent strengthening of central synapses is a key factor driving neuronal circuit behavior in the vertebrate CNS. At fast inhibitory synapses, strengthening is thought to occur by increasing the number of GABA(A) receptors (GABARs) of the same subunit composition to preexisting synapses. Here, we show that strengthening of mouse cerebellar granule cell GABAergic synapses occurs by a different mechanism. Specifically, we show that the neuropeptide hormone, insulin, strengthens inhibitory synapses by recruiting alpha 6-containing GABARs rather than accumulating more alpha 1-containing receptors that are resident to the synapse. Because alpha 6-receptors are targeted to functionally distinct postsynaptic sites from alpha 1-receptors, we conclude that only a subset of all inhibitory synapses are strengthened. Together with our recent findings on stellate cells, we propose a general mechanism by which mature inhibitory synapses are strengthened. In this scenario, alpha 1-GABARs resident to inhibitory synapses form the hardwiring of neuronal circuits with receptors of a different composition fulfilling a fundamental, but unappreciated, role in synapse strengthening.

• 出版日期2015-7-1
• 单位McGill