Atomic force microscopy captures folded ribosome bound nascent chains

作者：Loksztejn Anna^*; Scholl Zackary; Marszalek Piotr E

来源：Chemical Communications, 2012, 48(96): 11727-11729.

DOI：10.1039/c2cc35551e

Direct visualization of co-translational folding of nascent polypeptide chains is challenging. Here we present, for the first time, AFM images of large protein constructs based on the membrane binding domain of ankyrin-R, complexed with the ribosome. The characteristic "horse-shoe" shape of ankyrin-R emerging from the ribosome was captured.

• 出版日期2012