A multivariate analysis investigating different factors important for the interaction between liposomes and pectin

作者：Alund Siv Jorunn; Smistad Gro; Hiorth Marianne^*

来源：Colloids and Surfaces A: Physicochemical and Engineering Aspects , 2013, 420: 1-9.

DOI：10.1016/j.colsurfa.2012.11.079

• Liposome
• Pectin
• Coating
• Factorial design
• Multivariate analysis

Liposomes coated with a biopolymer such as pectin have a great potential in drug delivery. The drug formulation may be less toxic and in addition the mucoadhesive properties may be increased. The aim of this study was to investigate how different important factors affect the ability to produce single stable pectin coated liposomes. Experimental design and multivariate analysis were used in order to investigate many factors in a systematic manner by the use of a minimum of experiments. In a reduced factorial design, 2(4-1) design, the pectin concentration, lipid concentration, amount of charged lipid and the speed of addition under preparation were investigated. A central composite design investigated the three most important factors from the 2(4-1) design on three levels. The response variables in the studies, analysed by multivariate analysis, were the zeta potential and the change in particle size. These two parameters are important factors for verifying a successful coating of the liposomes. Summarizing the results from both designs revealed the most important factors for a successful coating, indicated by a high negative zeta potential, to be a high amount of charge in the lipid membrane and a low lipid to pectin ratio. The size of the coated liposomes was increased when the pectin concentration was high, indicating more complete covering of the liposomes. The size increased even more with a high lipid to pectin ratio probably due to bridging flocculation. To produce stable single pectin coated liposomes it is important to have enough pectin to cover the liposomes completely.

• 出版日期2013-3-5