Dietary isoleucine requirement of fingerling catla, Catla catla (Hamilton), based on growth, protein productive value, isoleucine retention efficiency and carcass composition

作者：Zehra Seemab; Khan Mukhtar A^*

来源：Aquaculture International, 2013, 21(6): 1243-1259.

DOI：10.1007/s10499-013-9627-8

• Catla catla
• Isoleucine requirement
• Growth
• Protein productive value

In order to determine the dietary isoleucine requirement of fingerling catla, Catla catla (4.25 +/- A 0.15 cm, 0.61 +/- A 0.04 g), six isonitrogenous (33.0 % crude protein) and isocaloric (13.7 kJ/g digestible energy) amino acid test diets containing casein, gelatin and l-crystalline amino acids with graded levels of isoleucine (0.5, 0.75, 1.0, 1.25, 1.5 and 1.75 % of the dry diet) were prepared. Triplicate groups of fish were randomly stocked in eighteen 70-l indoor polyvinyl circular troughs at a density of 25 fingerling per trough provided with a water flow-through system (1-1.5 l min(-1)). The experimental diets were fed to fish to apparent satiation at 08:00, 12:30 and 17:30 h for 12 weeks. Growth of the fish was found to increase with the incremental levels of dietary isoleucine up to 1.25 % of the dry diet. Quadratic regression analysis at 95 % maximum response of absolute weight gain (6.18 g fish(-1)), protein productive value (0.32), isoleucine retention efficiency (71.91 g fish(-1)), RNA/DNA ratio (4.81) and carcass protein (15.7 %) yielded the optimum isoleucine requirement in the range of 1.13-1.18 % of the dry diet, corresponding to 3.42-3.58 % of dietary protein. Data generated in this experiment would be useful to formulate isoleucine-balanced, cost-effective quality feeds for fingerling catla.

• 出版日期2013-12