A universal code for RNA recognition by PUF proteins

作者:Filipovska Aleksandra*; Razif Muhammad F M; Nygard Karoline K A; Rackham Oliver
来源:Nature Chemical Biology, 2011, 7(7): 425-427.
DOI:10.1038/NCHEMBIO.577

摘要

The design of proteins that can bind any RNA sequence of interest has many potential biological and medical applications. Here we have expanded the recognition of Pumilio and FBF homology protein (PUF) repeats beyond adenine, guanine and uracil and evolved them to specifically bind cytosine. These repeat sequences can be used to create PUF domains capable of selectively binding RNA targets of diverse sequence and structure.