Direct determination of serotonin in gut lavage fluid by liquid chromatographic ion trap tandem mass spectrometry

作者：Gregersen Kine; Froyland Livar; Berstad Amold; Araujo Pedro^*

来源：Talanta, 2008, 75(2): 466-472.

DOI：10.1016/j.talanta.2007.11.043

• serotonin
• liquid chromatography
• ion trap mass spectrometry
• uniform shell design
• gut lavage fluid

Direct determination of serotonin (5-HT) in gut lavage fluid from patients examined due to various gastrointestinal complaints has been achieved. The method involves addition of 5-methoxytryptamine (5-CH3O-HT) internal standard, centrifugation, filtration and injection of the sample supernatant in a liquid chromatographic system coupled to an ion trap tandem mass detector. Electro-spray in positive mode was used to isolate and fragment the protonated ions [5-HT + H](+) and [5-CH3O-HT +H](+) signals 177 and 191 nVz, respectively. Quantification was carried out by extracting the ion fragment chromatograms at 160 and 174m/z for 5-HT and 5-CH3O-HT, respectively. The relationship 5-HT/5-CH3O-HT was modelled by using a simultaneous design in order to estimate the optimal amount of internal standard to be added to the samples prior to quantification.

• 出版日期2008-4-15
• 单位河北医科大学