Dioxinodehydroeckol protects human keratinocyte cells from UVB-induced apoptosis modulated by related genes Bax/Bcl-2 and caspase pathway

作者：Ryu, BoMi; Aim, Byul-Nim; Kang, Kyong-Hwa; Kim, Young-Sang; Li, Yong-Xin; Kong, Chang-Suk; Kim, Se-Kwon^*; Kim, Dong Gyu

来源：Journal of Photochemistry and Photobiology B: Biology , 2015, 153: 352-357.

DOI：10.1016/j.jphotobiol.2015.10.018

• ULTRAVIOLET-B RADIATION
• ECKLONIA-CAVA
• PHOTOOXIDATIVE STRESS
• PHLOROTANNINS
• SKIN

Although ultraviolet B (UVB) has a low level of skin penetration, it readily results in epidermal sunburn of keratinocytes that are destined to apoptosis after sun expose, and leads to DNA damage.
Dioxinodehydroeckol (DHE), a phlorotannin from Ecklonia cava has been explored for its preventive activity against UVB-induced apoptosis in human keratinocyte (HaCaT) cells; however, the protective effects of treatment with low doses of DHE on UVB-damaged cells post-UVB exposure and their underlying mechanisms still remain unclear.
The HaCaT cells were exposed to 20 mJ cm(-2) of UVB irradiation which is the minimal erythema dose (MED) for individuals to be able to tan, and the expression levels of Bax/Bcl-2 and caspase-3,-8, -9 which are associated genes with apoptosis were investigated when we either treated cells with DHE doses after UVB irradiation or exposed them to UVB only.
Our results suggest insight into proposed mechanistic pathway of protective activity of DHE on the HaCaT cells from UVB-induced apoptosis, indicating the benefit of DHE as a repair agent for skin damage against UVB.

• 出版日期2015-12
• 单位上海市中医医院; 上海中医药大学