An improved method to measure lipase activity in aqueous media

作者：Hernandez Garcia Samanta^*; Inmaculada Garcia Garcia Maria; Garcia Carmona Francisco

来源：Analytical Biochemistry, 2017, 530: 104-106.

DOI：10.1016/j.ab.2017.05.012

• Lipase hydrolytic activity
• Pseudomonas fluorescens
• Mucor javanicus
• p-nitrophenyl esters
• Ethylene glycol
• p-nitrophenol

An improved method based on the p-nitrophenyl long chain esters method is proposed for measuring lipase hydrolytic activity in aqueous media. Using ethylene glycol as co-solvent for hydrophobic p-nitrophenyl substrates in aqueous buffer, lipase activity is measured by following the release of p-nitrophenol.
This fast and easy to handle method improves the solubility of both substrate and product, and also the stability of the substrate. It avoids the use of solvents such as ethanol or propanol, permits the comparison of all the p-nitrophenol acyl ester substrates and allows the influence of ions like Ca+2 to be studied, while avoiding turbidity in the reaction medium.

• 出版日期2017-8-1