Use of repetitive sequences and the polymerase chain reaction (rep-PCR) to fingerprint the genomes of Frankia isolates

作者：Murry MA^*; Zhang D; Schneider M; deBruijn FJ

来源：Symbiosis, 1995, 19(2-3): 223-240.

• Frankia
• rep-PCR
• genomic fingerprinting
• 16S rRNA

Oligonucleotide primers complimentary to consensus sequence motifs of repetitive elements common to prokaryotic genomes, REP (repetitive extragenic palindromic), ERIC (enterobacterial repetitive intergenic consensus), and BOX elements, were used to amplify intervening sequences of Frankia genomic DNA using the polymerase chain reaction (termed rep-PCR). The PCR reaction products were separated electrophoretically producing a complex banding pattern or fingerprint that was characteristic of each strain. Members of the same genomic species were found to display similar fingerprints, but the rep-PCR technique was sensitive enough to distinguish closely related strains and provides an effective means to rapidly differentiate between Frankia isolates at the sub-species level.

• 出版日期1995