Assessment of the expression of biomarkers of uremic inflammation in dogs with renal disease

作者：Nentwig Alice^*; Schweighauser Ariane; Maissen Villiger Carla; Bruckmaier Rupert M; Zurbriggen Andreas; van Dorland H Anette; Francey Thierry

来源：AJVR: American Journal of Veterinary Research , 2016, 77(2): 218-224.

DOI：10.2460/ajvr.77.2.218

OBJECTIVE
To assess the expression of inflammatory cytokines and enzymes in venous whole blood of dogs with impaired renal function attributable to various causes.
ANIMALS
46 dogs with acute kidney injury (AKI), 8 dogs with chronic kidney disease (CKD), and 10 healthy dogs.
PROCEDURES
Dogs with AKI and CKD were prospectively enrolled during 2010 if they met inclusion criteria. Demographic and laboratory characteristics were evaluated for each dog, and expression of inflammatory cytokines (interleukin [IL]-1 alpha, IL-1 beta, IL-8, tumor necrosis factor [TNF]-alpha, IL-10, and transforming growth factor [TGF]-beta) and enzymes (inducible nitric oxide synthase [iNOS] and 5-lipoxygenase [5-LO]) was measured in venous whole blood obtained at initial evaluation.
RESULTS
Dogs with impaired renal function had markedly higher expression of the cytokines IL-1 alpha, IL-1 beta, and TGF-beta and the enzyme 5-LO, compared with expression in healthy dogs. Additionally, 17 of 46 AKI dogs (but none of the CKD dogs) had higher IL-8 mRNA expression and 3 of 8 CKD dogs (but only 2/46 AKI dogs) had higher TNF-alpha expression, compared with results for healthy dogs. No significant difference between renal disease groups was detected for inflammatory markers and laboratory variables, degree of azotemia, or cause of impaired renal function.
CONCLUSIONS AND CLINICAL RELEVANCE
In this study, expression of the cytokines IL-1 alpha, IL-1 beta, and TGF-beta and the enzyme 5-LO was clearly increased in dogs with renal disease, which suggested that these markers were part of an inflammatory response in animals with AKI or CKD.

• 出版日期2016-2