Nanodiscs as a Modular Platform for Multimodal MR-Optical Imaging

作者：Carney Christiane E; Lenov Ivan L; Baker Catherine J; MacRenaris Keith W; Eckermann Amanda L; Sligar Stephen G^*; Meade Thomas J

来源：Bioconjugate Chemistry, 2015, 26(5): 899-905.

DOI：10.1021/acs.bioconjchem.5b00107

Nanodiscs are monodisperse, self-assembled discoidal particles that consist of a lipid bilayer encircled by membrane scaffold proteins (MSP). Nanodiscs have been used to solubilize membrane proteins for structural and functional studies and deliver therapeutic phospholipids. Herein, we report on tetramethylrhodamine (TMR) tagged nanodiscs that solubilize lipophilic MR contrast agents for generation of multimodal nanoparticles for cellular imaging. We incorporate both multimeric and monomeric Gd(III)-based contrast agents into nanodiscs and show that particles containing the monomeric agent (ND2) label cells with high efficiency and generate significant image contrast at 7 T compared to nanodiscs containing the multimeric agent (ND1) and Prohance, a clinically approved contrast agent.

• 出版日期2015-5
• 单位西北大学