Development of PCR for the Identification of Porcine Circovirus Type 2 (PCV-2) Genotype PCV-2a and PCV-2b

作者：He, Dongsheng^*; Zhao, Yanzong; Su, Danping; Niu, Xiaoyun

来源：Journal of Animal and Veterinary Advances, 2011, 10(18): 2398-2401.

• Porcine circovirus type 2 (PCV-2)
• porcine circovirus type 2a (PCV-2a)
• porcine circovirus type 2b (PCV-2b)
• agent
• target
• China

PCR was developed to evaluate for its ability to simultaneously detect viral infections of swine. Specific primers were designed for each sub-type of porcine cicovirus type 2 (PCV-2); porcine circovirus type 2a (PCV-2a) and porcine circovirus type 2b (PCV-2b). Each target produced specific amplicon with a size of 229 bp (PCV-2a) and 785 bp (PCV-2b). The assay was sensitive and specific in detecting the target agent in clinical specimens. In conclusion, the PCR has the potential to be useful for routine molecular diagnosis and epidemiology.

• 出版日期2011
• 单位华南农业大学