Analysis of interaction of Sendai virus V protein and melanoma differentiation-associated gene 5

作者：Sakaguchi Takemasa^*; Irie Takashi; Kuwayama Masaru; Ueno Tatsuya; Yoshida Asuka; Kawabata Ryoko

来源：Microbiology and Immunology, 2011, 55(11): 760-767.

DOI：10.1111/j.1348-0421.2011.00379.x

• MDA5
• Sendai virus-V protein

Sendai virus (SeV), a pneumotropic virus of rodents, has an accessory protein, V, and the V protein has been shown to interact with MDA5, inhibiting IRF3 activation and interferon-beta production. In the present study, interaction of the V protein with various IRF3-activating proteins including MDA5 was investigated in a co-immunoprecipitation assay. We also investigated interaction of mutant V proteins from SeVs of low pathogenicity with MDA5. The V protein interacted with at least retinoic acid inducible gene I, inhibitor of kappa B kinase epsilon and IRF3 other than MDA5. However, only MDA5 interacted with the V protein dependently on the C-terminal V unique (Vu) region, inhibiting IRF3 reporter activation. The Vu region has been shown to be important for viral pathogenicity. We thus focused on interaction of the V protein with MDA5. Point mutations in the Vu region destabilized the V protein or abolished the interaction with MDA5 when the V protein was stable. The V-R(320)G protein was highly stable and interacted with MDA5, but did not inhibit activation of IRF3 induced by MDA5. Viral pathogenicity of SeV is related to the inhibitory effect of the V protein on MDA5, but is not always related to the binding of V protein with MDA5.

• 出版日期2011-11