科研之友
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摘要
Although there is mounting evidence that human adipose-derived stem cells (hADSCs) therapy is benefit to the recovery of osteoarthritis, the efficacy and regulation mechanisms remain to be further studied. In this study, hADSCs labeled with lentivirus vector containing enhanced green fluorescent protein (FG12-hADSCs) were injected into articular cavity to investigate their effects on osteoarthritis treatment. New Zealand white rabbits were used as osteoarthritis model by excision of anterior cruciate ligament (ACL) of knee, medial meniscectomy and cartilage removal in the articular cavity. One week after modeling, the FG12-hADSCs were injected into the articular cavity of osteoarthritic rabbits. After 4 and 8 weeks of transplantation, the exercise capacity of the rabbits with osteoarthritis recovered (p < 0.05), and the proliferation of chondrocytes was more obvious in the experimental group compared with the control group. More importantly, the findings that human collogan II and nuclear antibody were stained positive indicated that FG12-hADSCs can survive and produce progeny of chondrocytes in articular cartilage. Furthermore, the level of IGF-1 in the experimental group was significantly increased (p < 0.05). Our results demonstrated that hADSCs were able to repair artilage injury, which might be due to the significant increase of IGF-1 in the joint fluid.
