A new rhodamine B based fluorometric chemodosimeter for Cu2+ ion in aqueous and cellular media

作者:Kempahanumakkagaari Suresh Kumar; Thippeswamy Ramakrishnappa*; Malingappa Pandurangappa
来源:Journal of Luminescence, 2014, 146: 11-17.
DOI:10.1016/j.jlumin.2013.09.010

摘要

A simple, sensitive and selective fluorescent chemo dosimeter rhodamine B phenyl hydrazide (RBPH) for Cu2+ was proposed. This probe is non fluorescent and colorless but exhibits fluorescent enhancement at 580 nm and displayed color change from colorless to pink for Cu2+ in the pH range 1-6. Fluorescence microscope experimental results reveals that this chemo sensor is cell permeable and can be used for fluorescence imaging of Cu2+ ions in living cells. This probe can detect Cu2+ with good linear relationships from 10 to 100 nM with r=0.99971 then limit of detection was found to be 0.015 nM with +/- 0.91% RSD at 10 nM concentrations. 2013 Elsevier B.V.

  • 出版日期2014-2