Discovery of Pantoea rodasii Strain ND03 that Produces N-(3-Oxo-hexanoyl)-L-homoserine Lactone

作者：Yunos Nina Yusrina Muhamad; Tan Wen Si; Mohamad Nur Izzati; Tan Pui Wan; Adrian Tan Guan Sheng; Yin Wai Fong; Chan Kok Gan^*

来源：Sensors (Switzerland), 2014, 14(5): 9145-9152.

DOI：10.3390/s140509145

• cell-to-cell communication
• mass spectrometry
• triple quadrupole liquid chromatography mass spectrometry
• quorum sensing
• N-(3-oxohexanoyl) homoserine-lactone (3-oxo-C6-HSL)
• Pantoea rodasii

Proteobacteria use quorum sensing to regulate target gene expression in response to population density. Quorum sensing (QS) is achieved via so-called signalling molecules and the best-studied QS signalling system uses N-acyl homoserine lactones (AHLs). This study aimed to identify and characterize the production of AHLs by a bacterium ND03 isolated from a Malaysian tropical rainforest waterfall. Molecular identification showed that ND03 is a Pantoea sp. closely related to Pantoea rodasii. We used Chromobacterium violaceum CV026, an AHL biosensor for preliminary AHL production screening and then used high resolution triple quadrupole liquid chromatography-mass spectrometry, to confirm that P. rodasii strain ND03 produced N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL). To the best of our knowledge, this is the first report for such a discovery in P. rodasii strain ND03.

• 出版日期2014-5