Development of a laccase-based flow injection electrochemical biosensor for the determination of phenolic compounds and its application for monitoring remediation of Kraft E1 paper mill effluent

作者：Freire RS; Duran N; Kubota LT^*

来源：Analytica Chimica Acta, 2002, 463(2): 229-238.

DOI：10.1016/S0003-2670(02)00417-8

• laccase
• biosensor
• environmental monitoring
• paper mill effluents
• remediatiom
• ozonization

This paper describes the development of a new system for amperometric determination of phenolic compounds, and its application for monitoring these compounds in paper mill effluent. The method was based on a flow system, a dialysis sampler, and a laccase-based biosensor. The performance of this system was investigated with respect to pH, ionic strength, working potential, and flow-rate dependence. The biosensor showed an excellent long-term stability allowing measurements for over than 3 months. The sensitivity of laccase-based biosensor was tested for phenol, p-chlorophenol, guaiacol and chloroguaiacol; the detector presented selective measurements of micromolar concentration of these compounds. The integration of a dialysis membrane sampling in the system protected the biosensor surface from fouling and gave independence of sample conditions that commonly influence the biosensor performance. These favorable characteristics allowed its application for direct measurements in complex media with no sample pretreatment. This ability was continued employing this system in a continuous analysis of phenolic compounds during the remediation of paper mill effluent by ozonization process.

• 出版日期2002-7-22