Activation of Endothelial Transient Receptor Potential C3 Channel Is Required for Small Conductance Calcium-Activated Potassium Channel Activation and Sustained Endothelial Hyperpolarization and Vasodilation of Cerebral Artery

作者：Kochukov Mikhail Y; Balasubramanian Adithya; Abramowitz Joel; Birnbaumer Lutz; Marrelli Sean P^*

来源：Journal of the American Heart Association, 2014, 3(4): e000913.

DOI：10.1161/JAHA.114.000913

• cerebrovascular circulation
• endothelium
• endothelium-derived factors
• ion channels
• vasculature

Background-Transient receptor potential C3 (TRPC3) has been demonstrated to be involved in the regulation of vascular tone through endothelial cell (EC) hyperpolarization and endothelium-dependent hyperpolarization-mediated vasodilation. However, the mechanism by which TRPC3 regulates these processes remains unresolved. We tested the hypothesis that endothelial receptor stimulation triggers rapid TRPC3 trafficking to the plasma membrane, where it provides the source of Ca2+ influx for small conductance calcium-activated K+ (SKCa) channel activation and sustained EC hyperpolarization.
Methods and Results-Pressurized artery studies were performed with isolated mouse posterior cerebral artery. Treatment with a selective TRPC3 blocker (Pyr3) produced significant attenuation of endothelium-dependent hyperpolarization-mediated vasodilation and endothelial Ca2+ response (EC-specific Ca2+ biosensor) to intraluminal ATP. Pyr3 treatment also resulted in a reduced ATP-stimulated global Ca2+ and Ca2+ influx in primary cultures of cerebral endothelial cells. Patch-clamp studies with freshly isolated cerebral ECs demonstrated 2 components of EC hyperpolarization and K+ current activation in response to ATP. The early phase was dependent on intermediate conductance calcium-activated K+ channel activation, whereas the later sustained phase relied on SKCa channel activation. The SKCa channel-dependent phase was completely blocked with TRPC3 channel inhibition or in ECs of TRPC3 knockout mice and correlated with increased trafficking of TRPC3 (but not SKCa channel) to the plasma membrane.
Conclusions-We propose that TRPC3 dynamically regulates SKCa channel activation through receptor-dependent trafficking to the plasma membrane, where it provides the source of Ca2+ influx for sustained SKCa channel activation, EC hyperpolarization, and endothelium-dependent hyperpolarization-mediated vasodilation.

• 出版日期2014-8