Microtubule proteins and their post-translational forms in the cerebrospinal fluid of patients with paraparesis associated with HTLV-I infection and in SH-SY5Y cells: An in vitro model of HTLV-I-induced disease

作者：Maldonado Horacio; Ortiz Riano Emilio; Krause Bernardo; Barriga Andres; Medina Fernando; Pando M Elsa; Alberti Carolina; Kettlun Ana M; Collados Lucia; Garcia Lorena; Cartier Luis; Antonieta Valenzuela M

来源：Biological Research, 2008, 41(3): 239-252.

• Tropical spastic paraparesis
• cerebrospinal fluid
• SH-SY5Y cells
• retraction
• tau
• Phosphorylated-tau forms
• tubulin
• acetyl-tubulin

HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is characterized by axonal degeneration of the corticospinal tracts. The specific requirements for transport of proteins and organelles to the distal part of the long axon are crucial in the corticospinal tracts. Microtubule dysfunction Could be involved in this disease, configuring in axonal transport disease. We measured tubulin and its post-translational modified forms (acetylated and tyrosinated) in CSF of patients and controls, as well as tau and its phosphorylated forms. There were no significant differences in the contents of tubulin and acetyl-tubulin between patients and controls; tyrosyl-tubulin was not detected. In HAM/TSP, tau levels were significantly reduced, while the ratio of PT(181)/total tau was higher in patients than in controls, this, being completely reduced different from what is reported in other neurodegenerative diseases. Phosphorylation at T(181) was also confirmed by Mass Spectrometry analysis. Western Blotting with monospecific polyclonal antibodies against PS(199), pT(205), pT(231), PS(262), PS 356, pS(396), pS(404) and pS(122) did not how differences in phosphorylation in these residues between patients and controls. Treating human SH-SY5Y neuroblastoma cells, a well-known ill vitro neurite retraction model, with culture supernatant of MT-2 cells (HTLV-I infected cell line that secretes the viral Tax protein) we observed neurite retraction and ail increase in tau phosphorylation at T(181). A disruption of normal phosphorylation of tau protein in T(181) could result in its dysfunction, contributing to axonal damage.

• 出版日期2008