Multi-center and multi-method evaluation of in vitro activities of ceftaroline against S. aureus

作者：Koeth Laura M^*; Apfalter Petra; Becker Karsten; Gesu Giovanni; Martinez Martinez Luis; Lahiri Sushmita D; Alm Richard A; Ambler Jane; Iaconis Joseph

来源：Diagnostic Microbiology and Infectious Disease, 2016, 85(4): 452-458.

DOI：10.1016/j.diagmicrobio.2016.05.003

• Ceftaroline
• Antimicrobial susceptibility testing
• Method comparison
• Broth microdilution
• Gradient diffusion
• Disk diffusion

This five-site study was performed to assess the reproducibility of ceftaroline MIC and disk results for Staphylococcus aureus. Three commercial broth microdilution, three gradient diffusion and ceftaroline 5 mu g disk diffusion methods were compared to a reference broth microdilution method against challenge isolates (n = 41) and isolates collected at four European sites (n = 30/site). For four MIC methods (Sensititre and three gradient diffusion methods), 99.0% of consolidated MIC results were within +/-1 dilution of the reference MIC. Categorical agreement rates based on EUCAST breakpoints for the challenge isolates were 75.6-100% and for disk testing were 78.0-92.7%. There was no clear distinction between isolates with MIC results of 1 and 2 mg/L with regard to variation in MIC or molecular genotyping results. The addition of an intermediate category for isolates with MIC results of 2 mg/L would help to identify these isolates as borderline susceptible/non-susceptible isolates.

• 出版日期2016-8
• 单位河北医科大学