ANALYSIS OF POLYMERASE CHAIN-REACTION PRODUCT BY CAPILLARY ELECTROPHORESIS AND ITS APPLICATION TO THE DETECTION OF SINGLE-BASE SUBSTITUTION IN GENES

作者：ARAKAWA H; UETANAKA K; MAEDA M; TSUJI A

来源：Journal of Chromatography A, 1994, 664(1): 89-98.

DOI：10.1016/0021-9673(94)80632-2

Capillary gel electrophoresis (CGE) was studied for the direct analysis of polymerase chain reaction (PCR) amplified samples. A low cross-linked polyacrylamide gel (3%T, 0.5%C) was used for CGE with treated and untreated silica capillaries. CGE showed high reproducibility and resolution in the separation of DNA fragments (ca. 100-1000 base pairs) produced by PCR. The CGE system was applied to the detection of an amplification refractory mutation system (ARMS) and PCR-restriction fragment length polymorphism (PCR-RFLP), which are detection methods of single base substitution in genes using PCR. With the CGE system, full automation of PCR product detection is feasible.

• 出版日期1994-3-25