Defining the Interaction of Human Soluble Lectin ZG16p and Mycobacterial Phosphatidylinositol Mannosides

作者：Hanashima Shinya; Goetze Sebastian; Liu Yan; Ikeda Akemi; Kojima Aikawa Kyoko; Taniguchi Naoyuki; Silva Daniel Varon; Feizi Ten; Seeberger Peter H; Yamaguchi Yoshiki^*

来源：ChemBioChem, 2015, 16(10): 1502-1511.

DOI：10.1002/cbic.201500103

• carbohydrate microarrays
• chemical synthesis
• lectins
• microarrays
• NMR spectroscopy
• phosphatidyl inositol mannoside

ZG16p is a soluble mammalian lectin that interacts with mannose and heparan sulfate. Here we describe detailed analysis of the interaction of human ZG16p with mycobacterial phosphatidylinositol mannosides (PIMs) by glycan microarray and NMR. Pathogen-related glycan microarray analysis identified phosphatidylinositol mono- and di-mannosides (PIM1 and PIM2) as novel ligand candidates of ZG16p. Saturation transfer difference (STD) NMR and transferred NOE experiments with chemically synthesized PIM glycans indicate that PIMs preferentially interact with ZG16p by using the mannose residues. The binding site of PIM was identified by chemical-shift perturbation experiments with uniformly N-15-labeled ZG16p. NMR results with docking simulations suggest a binding mode of ZG16p and PIM glycan; this will help to elucidate the physiological role of ZG16p.

• 出版日期2015-7-6