Modeling and experiment of shuttling speed effects on the OSTRYCH

作者：Chen Jyh Jian^*; Yang Yao Tsung

来源：Applied Thermal Engineering, 2011, 31(14-15): 2797-2807.

DOI：10.1016/j.applthermaleng.2011.05.005

• Polymerase chain reaction
• Shuttling speed
• Oscillatory thermocycler
• Heat transfer
• Continuous flow PCR

A novel Oscillatory TheRmal cYcler CHamber (OSTRYCH) has been designed and fabricated in our work. The sample is placed in a fixed chamber, and three constant isothermal zones are established and lined up in the system. The sample is oscillated and comes into contact with three different isothermal zones to complete thermal cycles.' The commercial software CFD-ACE+(TM) is utilized to investigate the influences of various chamber materials, boundary conditions and moving speeds of the chamber on the temperature distributions inside the chamber. The chamber moves at a specific speed and the boundary conditions with time variations are related to the moving speed. When the chamber moves, the boundary is specified at convection condition or the uniform temperature condition. The user subroutines coded and compiled by the FORTRAN language are introduced into the software to make the numerical results realistic. Results show that regarding the temperature profiles and the standard deviation of the temperature at the Y-cut cross section, the effects of various moving speeds of the chamber on the temperature distributions are negligible at the assigned time duration. The central temperatures of the chamber with various moving speeds are measured. The repeatability and stability of the OSTRYCH are examined. Finally, the experimental results and numerical simulations are compared and shown a similar trend.

• 出版日期2011-10