科研之友
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摘要
Background: It was previously shown that appropriate distribution of immune cells between different tissues and organs of the body is required for proper function of the immune system. Our previous experiments demonstrated that surgical trauma in mice induces T lymphocyte migration from peripheral blood to peritoneal lymphoid organs. T cells have regulatory activity as they suppress the cell-mediated immune response in vitro via TGF-. In the current study, we aim to evaluate the influence of surgery on both Ta and T lymphocyte distribution in patients that underwent operation.Materials and Methods: We investigated the percentage of T and T cells in peripheral blood of patients undergoing standard surgical procedures (gastric resection, colorectal resection, cholecystectomy, and strumectomy) before and 3 days after operation. The percentage of T and T cells was evaluated by FACS Canto II cytofluorimeter. Results: We showed that only major surgery located in the peritoneal cavity (gastric and colorectal surgery) decreases the percentage of T cells in peripheral blood as opposed to less traumatic surgery (strumectomy and cholecystectomy) which does not have such effect. However, significant reduction of T cells after operation was only observed in a group of patients that underwent gastrectomy. Additionally, we found that gastrectomy results in significant reduction of cumulative T CD4 and T CD25 lymphocyte counts. Conclusion: Surgery results in decreased percentage of T lymphocytes in peripheral human blood, and this correlates with the severity and location of the surgical trauma. This observation may help to predict postoperative recovery after gastroabdominal surgery.
- 出版日期2017
