Attenuation of Vaccinia Tian Tan Strain by Removal of Viral TC7L-TK2L and TA35R Genes

作者：Kan, Shifu^*; Wang, Yuhang; Sun, Lili; Jia, Peng; Qi, Yanxin; Su, Jiaqiang; Liu, Lei; Yang, Guohua; Liu, Liming; Wang, Zhuoyue; Wang, Jinhui; Liu, Guangchen; Jin, Ningyi; Li, Xiao; Ding, Zhuang

来源：PLos One, 2012, 7(2): e31979.

DOI：10.1371/journal.pone.0031979

• SMALLPOX VACCINE
• VIRUS ANKARA
• HOST-RANGE
• PROTEIN
• RECOMBINANT
• VIRULENCE
• SYSTEM
• MICE
• IMMUNOGENICITY
• NYVAC

Vaccinia Tian Tan (VTT) was attenuated by deletion of the TC7L-TK2L and TA35R genes to generate MVTT3. The mutant was generated by replacing the open reading frames by a gene encoding enhanced green fluorescent protein (EGFP) flanked by loxP sites. Viruses expressing EGFP were then screened for and purified by serial plaque formation. In a second step the marker EGFP gene was removed by transfecting cells with a plasmid encoding cre recombinase and selecting for viruses that had lost the EGFP phenotype. The MVTT3 mutant was shown to be avirulent and immunogenic. These results support the conclusion that TC7L-TK2L and TA35R deletion mutants can be used as safe viral vectors or as platform for vaccines.

• 出版日期2012-2-21
• 单位深圳出入境检验检疫局动植物检验检疫技术中心; 中国人民解放军军事医学科学院; 吉林省肿瘤医院; 吉林大学