A flow cytometry-based assay for measuring invasion of red blood cells by Plasmodium falciparum

作者：Bei Amy K; DeSimone Tiffany M; Badiane Aida S; Ahouidi Ambroise D; Dieye Tandakha; Ndiaye Daouda; Sarr Ousmane; Ndir Omar; Mboup Souleymane; Duraisingh Manoj T^*

来源：American Journal of Hematology, 2010, 85(4): 234-237.

DOI：10.1002/ajh.21642

Variability in the ability of the malaria parasite Plasmodium falciparum to invade human erythrocytes is postulated to be an important determinant of disease severity. Both the parasite multiplication rate and erythrocyte selectivity are important parameters that underlie such variable invasion. We have established a flow cytometry-based method for simultaneously calculating both the parasitemia and the number of multiply-infected erythrocytes. Staining with the DNA-specific dye SYBR Green I allows quantitation of parasite invasion at the ring stage of parasite development. We discuss in vitro and in vivo applications and limitations of this method in relation to the study of parasite invasion. Am. J. Hematol. 85:234-237, 2010.

• 出版日期2010-4