Apoptosis and apoptotic pathway in actinic prurigo by immunohistochemistry

作者：Cuevas Gonzalez Juan Carlos; Vega Memije Maria Elisa^*; Garcia Vazquez Francisco Javier; Rodriguez Lobato Erika; Farfan Morales Jose Eduardo

来源：Medicina Oral Patologia Oral y Cirugia Bucal, 2016, 21(1): E53-E58.

DOI：10.4317/medoral.20765

• Apoptosis
• actinic prurigo
• cheilitis actinic prurigo

Background: Actinic prurigo (AP) is an idiopathic photodermatosis, this entity requires exposure to UV-B and -A to develop lesions. Apoptosis is a physiological death program that can be initiated by a permanently active mechanism (extrinsic pathway) or irreparable damage (intrinsic pathway).
Material and methods: Descriptive study, the sample size comprised 64 paraffin blocks of tissue with a diagnosis of AP. In H&E-stained slides, the diagnosis of AP was corroborated, and 1-mu m-thick sections were processed for immunohistochemistry (IHC). A database was constructed with SPSS version 20, Inc., Chicago, IL, USA, and descriptive statistics were analyzed by X2 test and comparison of means.
Results: A total of 64 cases were processed, of which 40 (62.5%) were cheilitis AP and 24 (37.5%) were AP in the skin. Of the 40 cheilitis samples, 27 were positive for Bcl-2 and caspase 3 (67.5%), p53 was expressed in 30 (75%). Of the skin lesions, p53 and caspase 3 were expressed in 18 of 24 cases (75%), and 13 were positive for Bcl-2 (54%).
Conclusions: We propose that apoptosis is the last step in the type IV subtype a-b hypersensitivity response-activation of the intrinsic pathway indicates that external factors, such as UV-A and -B are the trigger.

• 出版日期2016-1