Differential distribution and enrichment of non-coding RNAs in exosomes from normal and Cancer-associated fibroblasts in colorectal cancer

作者：Herrera Mercedes; Llorens Carlos; Rodriguez Marta; Herrera Alberto; Ramos Ricardo; Gil Beatriz; Candia Antonio; Jesus Larriba Maria; Garre Pilar; Earl Julie; Rodriguez Garrote Mercedes; Caldes Trinidad; Bonilla Felix; Carrato Alfredo; Garcia Barberan Vanesa^*; Pena Cristina^*

来源：Molecular Cancer, 2018, 17(1): 114.

DOI：10.1186/s12943-018-0863-4

• Colon Cancer
• Liquid biopsy
• Tumor microenvironment
• Exosomes
• Non-coding RNAs
• Next generation sequencing

Exosome production from cancer-associated fibroblasts seems to be an important driver of tumor progression. We report the first in-depth biotype characterization of ncRNAs, analyzed by Next Generation Sequencing and Bioinformatics, expressed in established primary human normal and cancer-associated fibroblasts (CAFs) from cancer and normal mucosa tissues from 9 colorectal cancer patients, and/or packaged in their derived exosomes. Differential representation and enrichment analyses based on these ncRNAs revealed a significant number of differences between the ncRNA content of exosomes and the expression patterns of the normal and cancer-associated fibroblast cells. ncRNA regulatory elements are specifically packaged in CAF-derived exosomes, supporting a specific cross-talk between CAFs and colon cancer cells and/or other stromal cells, mediated by exosomes. These sncRNAs are potential biomarkers present in cancer-associated fibroblast-derived exosomes, which should thereby contribute to developing new non-invasive diagnostic, prognostic and predictive methods for clinical applications in management of cancer patients.

• 出版日期2018-8-3