Multilayered silk scaffolds for meniscus tissue engineering

作者：Mandal Biman B; Park Sang Hyug; Gil Eun S; Kaplan David L^*

来源：Biomaterials, 2011, 32(2): 639-651.

DOI：10.1016/j.biomaterials.2010.08.115

• Silk
• Meniscus
• Cartilage
• Tissue engineering
• Biomaterials

Removal of injured/damaged meniscus a vital fibrocartilaginous load-bearing tissue impairs normal knee function and predisposes patients to osteoarthritis Meniscus tissue engineering solution is one option to improve outcomes and relieve pain In an attempt to fabricate knee meniscus grafts three layered wedge shaped silk meniscal scaffold system was engineered to mimic native meniscus architecture The scaffolds were seeded with human fibroblasts (outside) and chondrocytes (inside) in a spatial separated mode similar to native tissue in order to generate meniscus-like tissue in vitro In chondrogenic culture in the presence of TGF-b3 cell-seeded constructs increased in cellularity and extracellular matrix (ECM) content Histology and Immunohistochemistry confirmed maintenance of chondrocytic phenotype with higher levels of sulfated glycosaminoglycans (sGAG) and collagen types I and II Improved scaffold mechanical properties along with ECM alignment with time in culture sug

• 出版日期2011-1