Determination of pyruvic acid concentration using a bioluminescence system from Photobacterium leiognathi

作者：Xuan, Guanhua; Lu, Xiaodong; Wang, Jingxue^*; Lin, Hong; Liu, Huihui

来源：Photochemical and Photobiological Sciences, 2015, 14(6): 1163-1168.

DOI：10.1039/c5pp00118h

• FLUORESCENCE CAPILLARY ANALYSIS
• LACTIC-ACID
• ORGANIC-ACIDS
• ELECTROPHORESIS
• BIOSENSOR
• SENSITIVITY
• URINE
• SERUM
• FLUID
• ASSAY

A novel, highly sensitive and selective bacterial luminescence method for the detection of pyruvic acid (PA) is reported here. This method is based on a reaction system catalyzed by lactate dehydrogenase (LDH) with the bacterial luciferase-FMN:NADH oxidoreductase bioluminescence system in vitro. The reduced nicotinamide adenine dinucleotide (NADH) involved in the LDH reaction system could be quantitatively analyzed by the bioluminescence system. A good linear relationship between the luminescence intensity and pyruvic acid concentration was exhibited within the range of 0.00014-0.001 mol l(-1), and the pyruvic acid detection limit was found to be 8.537 x 10(-5) mol l(-1). This method was successfully applied to the detection of PA in quail serum with a good recovery of over 70%.

• 出版日期2015
• 单位中国海洋大学