An ultrasensitive electrochemical immunosensor for the detection of CD146 based on TiO2 colloidal sphere laden Au/Pd nanoparticles

作者：Wang Yaoguang; Li Yueyun; Ma Hongmin; Guo Aiping; Du Bin; Yan Tao^*; Wei Qin

来源：Analyst, 2015, 140(10): 3557-3564.

DOI：10.1039/c5an00156k

An ultrasensitive electrochemical immunosensor for the detection of cluster of differentiation 146 antigen (CD146) based on TiO2 colloidal sphere laden Au/Pd nanoparticles (Au/Pd@TiO2) was developed. In this work, reduced graphene oxide-tetraethylene pentamine (rGO-TEPA) was applied as an electrode modifying material to modify the surface of a glassy carbon electrode (GCE). Au/Pd@TiO2 was used as the secondary- antibody (Ab(2)) label for the fabrication of the immunosensor. Amperometric response of the immunosensor for electrocatalytic reduction of hydrogen peroxide (H2O2) was recorded. Electrochemical impedance spectroscopy (EIS) proved that fabrication of the immunosensor was successful. The anti-CD146 primary antibody (Ab(1)) was immobilized on the rGO-TEPA modified GCE by a cross-linking reagent of glutaraldehyde (GA). With Ab(1) immobilized onto the rGO-TEPA modified GCE and Ab(2) linked with Au/Pd@TiO2, the immunosensor displayed a wide linear range (0.0050-20 ng mL(-1)), a low detection limit (1.6 pg mL(-1)), good reproducibility, good selectivity and acceptable stability. The designed sensing strategy may provide a potential application in the detection of other tumor markers.

• 出版日期2015
• 单位山东大学; 济南大学; 山东理工大学