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摘要
Capillary electrophoresis was employed to determine hydroxyl radicals trapped by benzoic acid in ascorbic acid/Fe2+ system. The pH values of the background buffer, electrolyte concentration, surfactant concentration and separation voltage were optimized. The impacts of substrate concentration and reaction time on the production of hydroxyl radicals were invenstigated using the optimized electrophoresis conditions. All the substances in the system were separated within 15 min; the linear range of reaction product (m-hydroxybenzoic acid), was 5x10(-6) mol/L to 1x10(-3) mol/L, and the detection limit was 2. 8x10(-7) mol/L. This method was applied to determine the ability of catalase, proanthocyanidins (PACs) and chlorogenic acid (CGA) for scavenging hydroxyl radical individually (IC50) and combined with each other in the serum medium. The result showed that catalase and PACs, catalase and CGA had synergistic effect in scavenging hydroxyl radical and this effect to catalase and PACs was also dose-dependent.
- 出版日期2013-10
- 单位江苏大学
