A Discussion of Molecular Biology Methods for Protein Engineering

作者：Zawaira Alexander^*; Pooran Anil; Barichievy Samantha; Chopera Denis

来源：Molecular Biotechnology, 2012, 51(1): 67-102.

DOI：10.1007/s12033-011-9448-9

• Molecular biology
• Protein engineering
• Mutagenesis
• Site-directed mutagenesis
• Insertion mutagenesis
• Deletion mutagenesis
• Polymerase chain reaction
• Molecular cloning
• Recombinant protein expression
• Recombinant plasmid

A number of molecular biology techniques are available to generate variants from a particular start gene for eventual protein expression. We discuss the basic principles of these methods in a repertoire that may be used to achieve the elemental steps in protein engineering. These include site-directed, deletion and insertion mutagenesis. We provide detailed case studies, drawn from our own experiences, packaged together with conceptual discussions and include an analysis of the techniques presented with regards to their uses in protein engineering.

• 出版日期2012-5