A Critical Role for IL-17RB Signaling in HTLV-1 Tax-Induced NF-kappa B Activation and T-Cell Transformation

作者：Lavorgna Alfonso; Matsuoka Masao; Harhaj Edward William^*

来源：PLoS Pathogens, 2014, 10(10): e1004418.

DOI：10.1371/journal.ppat.1004418

Human T-cell leukemia virus type 1 (HTLV-1) infection is linked to the development of adult T-cell leukemia (ATL) and the neuroinflammatory disease HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP). The HTLV-1 Tax protein functions as a potent viral oncogene that constitutively activates the NF-kappa B transcription factor to transform T cells; however, the underlying mechanisms remain obscure. Here, using next-generation RNA sequencing we identified the IL-25 receptor subunit IL-17RB as an aberrantly overexpressed gene in HTLV-1 immortalized T cells. Tax induced the expression of IL-17RB in an I kappa B kinase (IKK) and NF-kappa B-dependent manner. Remarkably, Tax activation of the canonical NF-kappa B pathway in T cells was critically dependent on IL-17RB expression. IL-17RB and IL-25 were required for HTLV-1-induced immortalization of primary T cells, and the constitutive NF-kappa B activation and survival of HTLV-1 transformed T cells. IL-9 was identified as an important downstream target gene of the IL-17RB pathway that drives the proliferation of HTLV-1 transformed cells. Furthermore, IL-17RB was overexpressed in leukemic cells from a subset of ATL patients and also regulated NF-kappa B activation in some, but not all, Tax-negative ATL cell lines. Together, our results support a model whereby Tax instigates an IL-17RB-NF-kappa B feed-forward autocrine loop that is obligatory for HTLV-1 leukemogenesis.

• 出版日期2014-10