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摘要
Contents We investigated whether the limited access to androgens during late prenatal period alters expression of steroidogenic enzymes involved in androgen production: 3-hydroxysteroid dehydrogenase/5-4 isomerase (3-HSD), cytochrome P450 17-hydroxylase/17,20-lyase (CYP17) and 17-hydroxysteroid dehydrogenase type 1 (17-HSD1) or type 3 (17-HSD3) in the foetal porcine gonads. Pregnant gilts were injected with anti-androgen flutamide (for seven days, 50mg/day/kg bw) or corn oil (control) starting at 83 (GD90) or 101 (GD108) gestational day. To assess 3-HSD, CYP17 and 17-HSD1 or 17-HSD3 expression, real-time PCR and immunohistochemistry were performed. In testes from flutamide-treated foetuses, increased 3-HSD and CYP17 mRNA expression was observed in the GD90 group, while decreased 3-HSD and 17-HSD3 mRNA expression and increased CYP17 mRNA expression were found in the GD108 group. CYP17 and 17-HSD3 were localized in Leydig cells. Following flutamide administration, the intensity of CYP17 immunostaining was higher in both treated groups, while 17-HSD3 intensity was lower in the GD108 group. In ovaries from flutamide-treated foetuses in the GD90 group, mRNA level for 3-HSD was elevated, but it was diminished for CYP17 and 17-HSD1. In the GD108 group, flutamide treatment led to lower mRNA level for 3-HSD but higher for CYP17. 3-HSD was found in granulosa cells, while CYP17 was localized within egg nests and oocytes of forming follicles. Following flutamide treatment, the intensity of 3-HSD and CYP17 immunostaining was higher in the GD90 and GD108 groups, respectively. Immunohistochemical staining for 3-HSD was restricted to the ovary. Concluding, diminished androgen action in the porcine foetal gonads during late gestation induces changes in steroidogenic enzymes expression, which may led to changes in gonadal function. However, it seems that androgens exert diverse biological effects depending on the gestational period.
- 出版日期2014-10
